splenocyte cell suspensions Search Results


single-cell suspensions of splenocytes  (Orient Bio Company)
90
Orient Bio Company single-cell suspensions of splenocytes
Cells were treated with anti-IL-10 mAb (5 ng/ml) or rat IgG (isotype control) for 2 h prior to LPS and DeinoLys treatment. ( A ) Extracellular cytokine levels in the cell culture supernatant were measured using commercial ELISA kits. ( B ) Surface molecules were measured by flow cytometry. The mean fluorescence intensity of each surface molecule in CD11c + cells is represented by bar graphs. ( C ) CD4 + and CD8 + T cells isolated from <t>splenocytes</t> of BALB/c mice were stained with CellTrace™ Violet Cell Proliferation Kit and cocultured with DCs treated with PBS (Con), DeinoLys, LPS, or LPS with DeinoLys in the presence or absence of anti-IL-10 mAb or rat IgG. The proliferation of CD4 + or CD8 + T cells was analyzed by flow cytometry. The mean SD ( n = 3) is shown in all bar graphs. One-way ANOVA was used for statistics, followed by Tukey's multiple comparison. * p < 0.05, ** p < 0.01, and *** p < 0.001.
Single Cell Suspensions Of Splenocytes, supplied by Orient Bio Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/single-cell suspensions of splenocytes/product/Orient Bio Company
Average 90 stars, based on 1 article reviews
single-cell suspensions of splenocytes - by Bioz Stars, 2026-03
90/100 stars
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splenocyte cell suspensions  (Charles River Laboratories)
90
Charles River Laboratories splenocyte cell suspensions
Cells were treated with anti-IL-10 mAb (5 ng/ml) or rat IgG (isotype control) for 2 h prior to LPS and DeinoLys treatment. ( A ) Extracellular cytokine levels in the cell culture supernatant were measured using commercial ELISA kits. ( B ) Surface molecules were measured by flow cytometry. The mean fluorescence intensity of each surface molecule in CD11c + cells is represented by bar graphs. ( C ) CD4 + and CD8 + T cells isolated from <t>splenocytes</t> of BALB/c mice were stained with CellTrace™ Violet Cell Proliferation Kit and cocultured with DCs treated with PBS (Con), DeinoLys, LPS, or LPS with DeinoLys in the presence or absence of anti-IL-10 mAb or rat IgG. The proliferation of CD4 + or CD8 + T cells was analyzed by flow cytometry. The mean SD ( n = 3) is shown in all bar graphs. One-way ANOVA was used for statistics, followed by Tukey's multiple comparison. * p < 0.05, ** p < 0.01, and *** p < 0.001.
Splenocyte Cell Suspensions, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/splenocyte cell suspensions/product/Charles River Laboratories
Average 90 stars, based on 1 article reviews
splenocyte cell suspensions - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Cells were treated with anti-IL-10 mAb (5 ng/ml) or rat IgG (isotype control) for 2 h prior to LPS and DeinoLys treatment. ( A ) Extracellular cytokine levels in the cell culture supernatant were measured using commercial ELISA kits. ( B ) Surface molecules were measured by flow cytometry. The mean fluorescence intensity of each surface molecule in CD11c + cells is represented by bar graphs. ( C ) CD4 + and CD8 + T cells isolated from splenocytes of BALB/c mice were stained with CellTrace™ Violet Cell Proliferation Kit and cocultured with DCs treated with PBS (Con), DeinoLys, LPS, or LPS with DeinoLys in the presence or absence of anti-IL-10 mAb or rat IgG. The proliferation of CD4 + or CD8 + T cells was analyzed by flow cytometry. The mean SD ( n = 3) is shown in all bar graphs. One-way ANOVA was used for statistics, followed by Tukey's multiple comparison. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Journal of Microbiology and Biotechnology

Article Title: Deinococcus radiodurans R1 Lysate Induces Tolerogenic Maturation in Lipopolysaccharide-Stimulated Dendritic Cells and Protects Dextran Sulfate Sodium-Induced Colitis in Mice

doi: 10.4014/jmb.2203.03008

Figure Lengend Snippet: Cells were treated with anti-IL-10 mAb (5 ng/ml) or rat IgG (isotype control) for 2 h prior to LPS and DeinoLys treatment. ( A ) Extracellular cytokine levels in the cell culture supernatant were measured using commercial ELISA kits. ( B ) Surface molecules were measured by flow cytometry. The mean fluorescence intensity of each surface molecule in CD11c + cells is represented by bar graphs. ( C ) CD4 + and CD8 + T cells isolated from splenocytes of BALB/c mice were stained with CellTrace™ Violet Cell Proliferation Kit and cocultured with DCs treated with PBS (Con), DeinoLys, LPS, or LPS with DeinoLys in the presence or absence of anti-IL-10 mAb or rat IgG. The proliferation of CD4 + or CD8 + T cells was analyzed by flow cytometry. The mean SD ( n = 3) is shown in all bar graphs. One-way ANOVA was used for statistics, followed by Tukey's multiple comparison. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: Following the established methodology, single-cell suspensions of splenocytes were obtained from 7-week-old BALB/c mice (Orient Bio, Inc.) [ ].

Techniques: Control, Cell Culture, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Fluorescence, Isolation, Staining, Comparison